Perfusion circuit

Perfusion circuit PCS3c

In the case of different culture media flowing through the upper and lower chambers, a multi-channel pump is used to give similar flow rates in both circuits. Alternatively, the flow from a single outlet from the media reservoir may be split by using Y-connectors. For convenience, both circuits may be merged after leaving the chamber using a Y-connector.

Perfusion circuit TCS2c

Different flow rates in the inner and outer circuits can be generated using different diameters of tubing. Using sterile couplings mounted between the Luer-connectors, the media bag can be changed without interrupting the media flow.

Perfusion circuit FCS1c

In this open circuit, the culture medium passes through the flow chamber at a shear rate defined by the pump and is collected in another reservoir. The culture medium is pre-conditioned in a gas-permeable media bag by equilibrating in a CO2 incubator. Using impermeable tubing, the partial gas pressure remains stable until the medium reaches the cell monolayer.