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culture media

cell culture handling

Passage kitSerum contains trypsin inhibitors. Thus it is important to remove traces of serum using solution 1. The incubation time for the trypsin (solution 2 of passage kit 1 & 2) and the degree of force required to get the cells into single cell suspension varies between cell types. Solution 3 will inactivate the remaining trypsin and prevent cell damage. Since solution 3 (of passage kit 2) contains soybean trypsin inhibitor, it may be used for cultures in serum-free medium, too.

Passage kits 1 & 2 are recommended for the cell types listed below:

    → Endothelial cells, chondrocytes, osteoblasts, periost cells, fibroblasts, myocytes, epithelial cell

Passage kit 3 contains dispase to detach cells highly sensitive to trypsin and is recommended for:

    → Keratinocytes, melanocytes

Passage kit 4 was developed as an universal and easy passage kit. It consist of a recombinant enzyme as detachment solution. To use a neutralising solution is not necessary. It is recommended for:

    → All adherent cell types

Cryo solution

Cryopreservation is a process of preserving cells by cooling to low sub-zero temperatures. At these low temperatures, any biological activity is effectively stopped. However, when cryo solutions are not used, the cells being preserved are often damaged due to freezing during the approach to low temperatures or warming to room temperature. Phenomena which can cause damage to cells during cryopreservation are solution effects, extracellular ice formation, dehydration and intracellular ice formation.

Supplement kits

Provitro’s cell culture media are supplied in several variations of unit assembly system. Among those, we offer culture media with individual vials for each single supplement as kit version. If you need flexibility in your experiments, we are able to provide you with supplement kit that enables you only to add that supplement you really want to give into your basal culture medium.

Reagents of cell culture
OFFER NO.PRODUCTSPECIFICATIONPDF
204 000150 ml PBS + 25 ml Tryp./EDTA + 25 ml neutral.sol. (with FCS)Passage kit 1download
204 000250 ml PBS + 25 ml Tryp./EDTA + 25 ml neutral.sol. (serum-free)Passage kit 2download
204 000350 ml PBS + 25 ml Dispase II solutionPassage kit 3download
204 000450 ml PBS + 25 ml detachment solutionPassage kit 4download
204 0101Cryo solution, 125 ml (serum-free)Cryo-SFMdownload
204 0102Cryo solution, 30 ml (serum-free)Cryo-SFMdownload
204 3100BIT-100 supplement On request
Supplement kit for culture media
OFFER NO.PRODUCTSPECIFICATIONPDF
215 0001Supplement kit for endothelial cell proliferation mediumFCS kitdownload
216 0001Supplement kit for endothelial cell proliferation mediumHuS kitdownload
215 1101Supplement kit for endothelial cell growth medium, advancedFCS kitdownload
216 1101Supplement kit for endothelial cell growth medium, advancedHuS kitdownload
215 0102Supplement kit for microvascular endothelial cell growth mediumFCS kitdownload
216 0102Supplement kit for microvascular endothelial cell growth mediumHuS kitdownload
215 1102Supplement kit for microvascular endothelial cell growth medium, advancedFCS kitdownload
216 1102Supplement kit for microvascular endothelial cell growth medium, advancedHuS kitdownload
217 0401Supplement kit for defined fibroblast maintenance mediumSerum-free kitdownload
217 0502Supplement kit for melanocyte growth mediumSerum-free kitdownload
215 0601Supplement kit for smooth muscle cell growth mediumFCS kitdownload
216 0601Supplement kit for smooth muscle cell growth mediumHuS kitdownload
215 0602Supplement kit for skeletal muscle cell growth mediumFCS kitdownload
216 0602Supplement kit for skeletal muscle cell growth mediumHuS kitdownload
217 0603Supplement kit for skeletal muscle cell differentiation mediumSerum-free kitdownload