|Circuit part list|
|300 0101||Silicone tubing, Luer fitting on each side||i.d = 1 mm; l = 1 m|
|300 0102||Silicone tubing, Luer fitting on each side||i.d = 3 mm; l = 1 m|
|300 0210||Luer tubing adapter, male and female, 5 each||i.d. = 1.6 mm|
|300 0220||Luer tubing adapter, male and female, 5 each||i.d. = 3.2 mm|
|300 0303||Gas-permeable media bag||V = 1000 ml|
|300 0400||Sterile Luer coupling, pack of 10|
|300 0511||Pump ISMATEC IPC-4||0.002 ... 44 ml/min|
|300 0512||Pump ISMATEC IPC-N4||0.0004 ... 11 ml/min|
|300 0513||Pump ISMATEC IPC-8||0.002 ... 44 ml/min|
|300 0514||Pump ISMATEC IPC-N8||0.0004 ... 11 ml/min|
|300 0521||Pump tubing, with 2 stoppers, Luer fitting on each side||i.d. = 1 mm|
|300 0522||Pump tubing, with 2 stoppers, Luer fitting on each side||i.d. = 3 mm|
|300 0710||Y-connector, pack of 2||i.d. = 1.6 mm|
|300 0720||Y-connector, pack of 2||i.d. = 3.2 mm|
Perfusion circuit PCS3c
In the case of different culture media flowing through the upper and lower chambers, a multi-channel pump is used to give similar flow rates in both circuits. Alternatively, the flow from a single outlet from the media reservoir may be split by using Y-connectors. For convenience, both circuits may be merged after leaving the chamber using a Y-connector.
Perfusion circuit TCS2c
Different flow rates in the inner and outer circuits can be generated using different diameters of tubing. Using sterile couplings mounted between the Luer-connectors, the media bag can be changed without interrupting the media flow.
Perfusion circuit FCS1c
In this open circuit, the culture medium passes through the flow chamber at a shear rate defined by the pump and is collected in another reservoir. The culture medium is pre-conditioned in a gas-permeable media bag by equilibrating in a CO2 incubator. Using impermeable tubing, the partial gas pressure remains stable until the medium reaches the cell monolayer.