Cell culture handling

Passage kitSerum contains trypsin inhibitors. Thus it is important to remove traces of serum using solution 1. The incubation time for the trypsin (solution 2 of passage kit 1 & 2) and the degree of force required to get the cells into single cell suspension varies between cell types. Solution 3 will inactivate the remaining trypsin and prevent cell damage. Since solution 3 (of passage kit 2) contains soybean trypsin inhibitor, it may be used for cultures in serum-free medium, too.

Passage kits 1 & 2 are recommended for the cell types listed below:

  • Endothelial cells, chondrocytes, osteoblasts, periost cells, fibroblasts, myocytes, epithelial cell

Passage kit 3 contains dispase to detach cells highly sensitive to trypsin and is recommended for:

  • Keratinocytes, melanocytes

NEW!!! Passage kit 4 was developed as an universal and easy passage kit and is recommended for all cell types. It consist of an recombinant enzyme as detachment solution. To use a neutralising solution is not nescessary.

Cryo solution

Cryopreservation is a process of preserving cells by cooling to low sub-zero temperatures. At these low temperatures, any biological activity is effectively stopped. However, when cryo solutions are not used, the cells being preserved are often damaged due to freezing during the approach to low temperatures or warming to room temperature. Phenomena which can cause damage to cells during cryopreservation are solution effects, extracellular ice formation, dehydration and intracellular ice formation.

Supplement kits

Provitro’s cell culture media are supplied in several variations of unit assembly system. Among those, we offer culture media with individual vials for each single supplement as kit version. If you need flexibility in your experiments, we are able to provide you with supplement kit that enables you only to add that supplement you really want to give into your basal culture medium.

Reagents of cell culture
OFFER NO PRODUCT SPECIFICS DETAILS
204 0001 50 ml PBS + 25 ml Tryp./EDTA + 25 ml neutral.sol. (with FCS) Passage kit 1
204 0002 50 ml PBS + 25 ml Tryp./EDTA + 25 ml neutral.sol. (serum-free) Passage kit 2
204 0003 50 ml PBS + 25 ml Dispase II solution Passage kit 3
204 0004 50 ml PBS + 25 ml detachment solution Passage kit 4
204 0101 Cryo solution, 125 ml (serum-free) Cryo-SFM
204 0102 Cryo solution, 30 ml (serum-free) Cryo-SFM
204 3100 BIT-100 supplement
Supplement kit for culture media
OFFER NO PRODUCT SPECIFICS DETAILS
215 0101 Supplement kit for endothelial cell growth medium FCS kit
216 0101 Supplement kit for endothelial cell growth medium HuS kit
215 1101 Supplement kit for endothelial cell growth medium, advanced FCS kit
216 1101 Supplement kit for endothelial cell growth medium, advanced HuS kit
215 0102 Supplement kit for microvascular endothelial cell growth medium FCS kit
216 0102 Supplement kit for microvascular endothelial cell growth medium HuS kit
215 1102 Supplement kit for microvascular endothelial cell growth medium, advanced FCS kit
216 1102 Supplement kit for microvascular endothelial cell growth medium, advanced HuS kit
217 0401 Supplement kit for defined fibroblast maintenance medium Serum-free kit
217 0502 Supplement kit for melanocyte growth medium Serum-free kit
215 0601 Supplement kit for smooth muscle cell growth medium FCS kit
216 0601 Supplement kit for smooth muscle cell growth medium HuS kit
215 0602 Supplement kit for skeletal muscle cell growth medium FCS kit
216 0602 Supplement kit for skeletal muscle cell growth medium HuS kit
217 0603 Supplement kit for skeletal muscle cell differentiation medium Serum-free kit
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