Nucleic acid processing

Provitro’s quality management certified lab offers services for total RNA isolation from cells and various tissue types. Total RNA is isolated from your material, using the QIAGEN RNeasyTM system. Purified RNA will be aliquoted as necessary for further investigation according to the customer’s need and will be stored clearly labelled at -80°C. All processes are accomplished according to standard operation procedures (SOP). Quality control and product release are arranged according to clearly defined criteria.

  • Standard quality control
    Standard quality control is run for the purpose of checking on the ratio between absorbance values at 260 and 280 nm. That ratio provides for an estimate of the purity of RNA with regard to contaminants absorbed in the UV, such as proteins. The results allow to quantify the total amount of purified RNA and reveal possible sample contaminations detectable in the UV-range.
  • Advanced quality control
    On request, each sample may be tested regarding RNA degradation or signs of apoptose, using the Agilent 2100 Bioanalyser System. The resulting electropherogramme is checked for baseline development, marker peak and ribosomal signal intensity (ratio 28S/18S RNA).
Nucleic acid processing
OFFER NO PRODUCT
906 0101 RNA extraction from primary cells, cells not included
906 0102 DNA extraction from primary cells, cells not included
906 0201 RNA extraction from cell lines, cells not included
906 0202 DNA extraction from cell lines, cells not included
906 0311 Provisional gene targeting by RNAi in primary cells
906 0312 Provisional gene targeting by RNAi in cell lines
906 1211 Manufacturing and provision of 1 FFPE tissue slide, normal tissue
906 1221 Manufacturing and provision of 1 cryo tissue slide, normal tissue
906 2211 Manufacturing and provision of 1 FFPE tissue slide, pathological tissue
906 2221 Manufacturing and provision of 1 cryo tissue slide, pathological tissue
906 0501 RNA extraction from FFPE tissue
906 0502 DNA extraction from FFPE tissue
906 0601 Microdissection of relevant tissue area
906 0701 FISH analysis
906 1501 RNA extraction from FFPE tissue including quality control
906 1502 DNA extraction from FFPE tissue including quality control
906 1511 RNA extraction from cryo tissue including quality control
906 1512 DNA extraction from cryo tissue including quality control
906 2501 RNA quality control
906 2502 DNA quality control

*FFPE - formalin fixed, paraffin embedded

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