Perfusion circuit

Perfusion circuit PCS3c

In the case of different culture media flowing through the upper and lower chambers, a multi-channel pump is used to give similar flow rates in both circuits. Alternatively, the flow from a single outlet from the media reservoir may be split by using Y-connectors. For convenience, both circuits may be merged after leaving the chamber using a Y-connector.

Perfusion circuit TCS2c

Different flow rates in the inner and outer circuits can be generated using different diameters of tubing. Using sterile couplings mounted between the Luer-connectors, the media bag can be changed without interrupting the media flow.

Perfusion circuit FCS1c

In this open circuit, the culture medium passes through the flow chamber at a shear rate defined by the pump and is collected in another reservoir. The culture medium is pre-conditioned in a gas-permeable media bag by equilibrating in a CO2 incubator. Using impermeable tubing, the partial gas pressure remains stable until the medium reaches the cell monolayer.

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Circuit part list
OFFER NO PRODUCT SPECIFIC
300 0101 Silicone tubing, Luer fitting on each side i.d = 1 mm; l = 1 m
300 0102 Silicone tubing, Luer fitting on each side i.d = 3 mm; l = 1 m
300 0210 Luer tubing adapter, male and female, 5 each i.d. = 1.6 mm
300 0220 Luer tubing adapter, male and female, 5 each i.d. = 3.2 mm
300 0303 Gas-permeable media bag V = 1000 ml
300 0400 Sterile Luer coupling, pack of 10
300 0511 Pump ISMATEC IPC-4 0.002 ... 44 ml/min
300 0512 Pump ISMATEC IPC-N4 0.0004 ... 11 ml/min
300 0513 Pump ISMATEC IPC-8 0.002 ... 44 ml/min
300 0514 Pump ISMATEC IPC-N8 0.0004 ... 11 ml/min
300 0521 Pump tubing, with 2 stoppers, Luer fitting on each side i.d. = 1 mm
300 0522 Pump tubing, with 2 stoppers, Luer fitting on each side i.d. = 3 mm
300 0530 Pump-control software, free with pump ISMATEC IPC
300 0710 Y-connector, pack of 2 i.d. = 1.6 mm
300 0720 Y-connector, pack of 2 i.d. = 3.2 mm
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Perfusion culture systems